Objective To investigate the expression of WSIP-2 protein in human brain astrocytoma and its clinicopathological significance. Methods Expression of WSIP2 in 10 specimens of normal human brain tissue and 54 of human astrocytoma was examined by immunohistochemical staining. The relationship between the expression of WSIP-2 protein and the clinicopathological features in human brain astrocytoma was analyzed. Results The expression of WSIP2 was negative in all specimens of normal human brain tissue, and positive in 35 of 54 of human astrocytoma with a positive rate of 64.8%. The expression of WSIP-2 protein in astrocytoma specimens of grade ⅢⅣ was significantly higher than that of grade Ⅰ、Ⅱ(P<0.01). The increased expression of WSIP2 was positively correlated with the differentiation, size and recurrence of the tumor (P<0.01). Conclusion The overexpression of WSIP2 may be involved in the pathogensis and development of astrocytoma. The detection of the expression level of WSIP2 is helpful to evaluate the prognosis of the patients with this tumor.