OBJECTIVE:To investigate the repairing effect on nasal cartilage defect with associated graft of perichondrium and allogenic demineralized bone matrix (DBM). METHODS:Eighteen New Zealand rabbits were randomly divided into 3 groups (in each group, n=6). Nasal alar cartilage and perichondrium were ablated in each rabbit. Autogenic auricular perichondrium wrapped by allogenic DBM was grafted into the defect in group A, autogenic auricular cartilage in group B and autogenic auricular perichondrium in group C. At the 10th week after repair, the effect was evaluated by gross inspection, histopathology, immunohistochemical detection of Soluble protein100 (S100) and transforming growth factorβ1 (TGFβ1) antibodies of the cartilage from the defect. RESULTS:The cartilage cell and matrix from group A and B were similar to those of the normal cartilage with no obvious differences between group A and group B. Being compared with the cartilage cells from group A and group B, the cell number, cytoplasm, and positive S100 and TGFβ1 cells were significantly reduced in group C (P< 0.01). CONCLUSION:The repairing effect on nasal cartilage defect with associated graft of perichondrium and allogenic DBM is coincide with autogenic cartilage and surpass the autogenic perichondrium.