免疫性脑脊髓膜炎模型中听神经脱髓鞘病变与听觉诱发电位的变化
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仇志强,Email:zhiqiangchou@sohu.com

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甘肃省自然科学基金项目(编号0710RJZA035)


Change of demyelinating disease in auditory nerve pathology and auditory brainstem response in mice with autoimmune auditory nerve encephalomyelitis
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    摘要:

    摘 要:目的 探索实验性免疫性脑脊髓膜炎模型中听神经脱髓鞘病变和ABR随时间的变化,以及这两种变化之间的关系。方法 选取Wistar大鼠60只,随机分为实验组、对照组和正常组,分别皮下注射牛坐骨神经髓鞘碱蛋白和完全弗氏佐剂、完全弗氏佐剂、生理盐水。对所有实验动物在免疫前、免疫结束后1、2、3、4、5、6周进行ABR检查。每周随机抽取各组2只动物,2.5%戊二醛活体灌注后处死,电镜下观察听神经的变化。结果 实验组动物听神经在给药后1周均出现脱髓鞘病变,给药后第3周达到高峰,随后自行缓解,在第6周基本恢复;ABR的阈值各组间无明显差异,实验组Ⅲ波潜伏期延长。结论 免疫性脑脊髓膜炎模型中听神经的脱髓鞘病变发病后随时间自行缓解,与ABR的变化并不同步。

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    Abstract:Objective To study the pathological changes of auditory nerve and alteration of auditory brainstem response (ABR) in mice with experimental autoimmune encephalomyelitis (EAE), and the correlation between them. Methods Sixty Wistar mice were randomly divided into experimental group, control group and normal group. The animal model of EAE was established by subcutaneous injetion of heterologous myelin basic protein (MBP) of peripheral nerve and complete Freunds adjuvant. Antigens were replaced by complete Freunds adjuvant and normal saline in the normal group and control group respectively. ABR was examined before and weekly after immunization for seven weeks’ duration. Two rats of every group were killed weekly to observe the pathological changes of auditory nerve under electronic microscope. Results Demyelination was observed in the auditory nerve of experimental group at the first week after immunization, reached the peak at the third week, and got recovery at the 6th week with selfrelief. Except prolonged latency of wave Ⅲ in experimental group, the differences of ABR thresholds among the three groups were statistically insignificant. Conclusion Auditory nerve demyelination in the model mice with EAE can be eased up with time and ABR will not change synchronously.

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陈小婉 仇志强 马春霞 张小兵.免疫性脑脊髓膜炎模型中听神经脱髓鞘病变与听觉诱发电位的变化[J].中国耳鼻咽喉颅底外科杂志,2010,16(4):241-245

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  • 在线发布日期: 2010-08-31
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