目的 探讨喉癌组织中Notch1蛋白的表达及与患者临床病理资料的关系和对预后的影响。方法 采用免疫组化Envision二步法检测62例喉癌标本中Notch1的表达，同时选取15例不典型增生、10例声带白斑、l0例声带息肉标本作对照，结合患者临床病理资料分别进行分析。结果 62例喉癌组织中Notch1蛋白高表达率61.3%（38/62），低表达38.7%24/62），其高表达率与不典型增生、声带白斑及声带息肉相比差异均有统计学意义（P<0.01），Notch1高表达与T分级、临床分期及病理分级显著相关（P<0.05）。单因素分析Notch1高表达组总生存率较低表达组高（P<0.05），无瘤生存率两组差异无统计学意义（P>0.05），多因素分析复发、临床分期及Notch1表达情况对生存率影响较大，复发、高分期患者和Notch1低表达组生存期短。结论 Notch1在喉癌发生、发展中起重要作用，在评估喉癌预后方面有一定价值。
Abstract:Objective To investigate the expression of Notch1 in laryngeal squamous cell carcinoma (LSCC), while determine the relationship with clinicopathologic characteristics and prognosis. Methods Expression of Notch1 in 62 specimens of laryngeal squamous cell carcinoma and 35 control tissues (15 cases of atypical hyperplasia, 10 cases of leukoplakia of vocal cord and 10 cases of polyp of vocal cord) was examined by immunohistochemical staining (Envision DAKO). The relationship between the expression of Notch1 and the clinicopathological features in LSCC was analysed. Results Overexpression of Notch1 was detected in 38 specimens (61.3%), the rate of overexpressions in LSCC was significantly higher than that in atypical hyperplasia, leukoplakia and polyp of vocal cord (P<0.01). Overexpression of Notch1 was significantly correlated with T stage, clinical stage and pathological classification (P<0.05). With univariate analysis, overexpression of Notch1 was significantly associated with a better overall survival (P<0.05), but there was no significant difference in diseasefree survival (P>0.05). With multivariate analysis, recurrence, advanced stage and low expression of Notch1 were unfavorable prognostic factors. Conclusion The expression of Notch1 plays an important role in the process of tumorigenesis and development of LSCC, and has potential value in prognostic evaluation.
徐成志 董频 王珮华 阎小军. Notch1在喉癌组织中的表达及意义[J].中国耳鼻咽喉颅底外科杂志,2011,17(6):401-405复制