Objective To investigate the effect of epithelialmesenchymal transition (EMT) induced by transforming growth factorβ1 (TGF-β1) cotreated with epithelial growth factor (EGF) on invasive ability of human laryngeal carcinoma cell Hep-2. Methods Human laryngeal carcinoma cell line Hep-2 was treated with TGF-β1 or TGF-β1+EGF. Cellular morphological changes were observed seperatively under inverted microscope 24 h and 48 h after treatment. RT-PCR and Western blot assay were used for estimating several invasive genes: E-cadherin and vimentin. The invasive ability was detected by transwell chamber. Results ① EMT in Hep-2 was promoted both in the TGF-β1 group (48 h) and the TGF-β1+EGF group (24 and 48 h). ② The enhancement of Hep-2 invasive ability in TGF-β1+ EGF group (24 h and 48 h) were more obvious than that in TGFβ1 group（P<0.05). The invasive ability of TGF-β1 group (48 h) was more obvious than that of the control group (P<0.05). ③ E-caclherin expression decreased and Vimentin expression increased obviously in TGFβ1+EGF group. Conclusion EGF can promote EMT while co-treated with TGF-β1, and therefore enhance the invasive ability of human laryngeal carcinoma cell line Hep-2.