Objective To study the expression of pituitary tumor transforming gene (PTTG) in laryngeal squamous cell carcinoma (LSCC) and its clinical significance. Methods The expression of PTTG was detected with immunohistochemical SP method in 56 samples of LSCC and 13 of normal laryngeal mucosa. The relationship of expression of PTTG with the biological behaviors of LSCC was analyzed. Results The positive rates of PTTG were 64.3% and 23.1%in LSCC and normal laryngeal mucosa (P<0.05). The positive rate and the strength of expression of PTTG were statistically related with the biological behaviors of LSCC. Conclusion The expression of PTTG and its strength may become important targets in determining the biological behaviors and the prognosis for LSCC.