Objective To identify the existence of stem cells in mouse laryngeal mucosa by using 5bromodeoxyurdine (5-BrdU) labeling for the preliminary study. Methods 36 threedays Kunming white mice were randomly divided into group A, B and C. 5-BrdU of 100 μg/g and 50 μg/g were intraperitoneally injected to mice of group A and group B respectively, and normal saline to those of group C. All the mice were executed 8 weeks after injection. Immunohistochemical staining was applied to detect the labelretaining cells (LRCs) in the laryngeal mucosa, and the percentages of LRCs were calculated. Results There were LRCs in scalyepithelium of both group A and B, and the difference between the two groups was statistically insignificant (t=0.06, P=0.949). The expression of LRCs was not observed in group C. Conclusion There are LRCs in the mouse laryngeal mucosa. It is possible that stem cells exist in these LRCs. 