ObjectiveTo study the diagnosis and treatment of multifocal Warthin’s tumor located in parotid glands via analyzing its clinical characteristics.MethodsClinical data of 26 patients suffering from multifocal Warthin’s tumor located in parotid glands and treated in our department from 1991 to 2010 was analyzed retrospectively. The proper treatment strategies were evaluated via Fisher’s exact propability univariate analysis about relapse of the tumor.ResultsIn the 26 cases, the male/female ratio was 2.25∶1, and their onset ages ranged from 45 to 81 years. The accuracy of fine needle aspiration cytology (FNAC) was 84.4%. 23 patients (29 sides) underwent surgical treatment and 5 cases (6 sides) recurred postoperatively. Age, sex, smoking history, duration of symptoms, number, diameter and location of tumors, synchronous tumors, surgical approach, operative complications and postoperative smoking were pooled and analyzed. The analytic results showed that surgical approach was the independent risk factor for the recurrence of the tumor.ConclusionsThe clinical features of multifocal Warthin’s tumor of parotid glands are characteristic. FNAC should be performed routinely to confirm the diagnosis and surgical resection of gland lobe is a reasonable manner to prevent or reduce relapse of the tumor.