ObjectiveTo observe the effect of acupuncture at Neitinggong acupoint on the gentamycininduced deafness.Methods20 rats were randomly divided into 4 groups of five. Gentamycin group received an intraperitoneal injection of gentamycin every morning in a dose of 100 mg/kg for 14 consecutive days. Neitinggong group and Tinggong group received intraperitoneal injection of gentamycin as the same as the Gentamycin group, and were given acupuncture at Neitinggong or Tinggong separately once every two days from the first day of injection for a total of 10 times. Control group received intraperitoneal injection of normal saline of the same volume of gentamycin every morning without other interventions. Auditory brainstem response (ABR) examination was performed in all animals at day one, the 14th day and the 20th day to analyze their ABR thresholds.ResultsThe ABR threshold of Gentamycin group was significantly higher than those of the Neitinggong group, the Tinggong group and the control group separately. The difference of ABR threshold between the Neitinggong group and the Tinggong group was statistically insignificant.ConclusionsAcupuncture at Neitinggong and Tinggong acupoints can decrease the auditory brainstem response threshold and therefore reduce gentamycininduced ototoxicity to some extent, which indicates that the Neitinggong acupoint may be an effective acupoint for treatment of gentamycininduced deafness.