CD44作为筛选下咽癌肿瘤干细胞分子标志物的价值
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向明亮, Email: xiangming_liang@yahoo.com.cn

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国家自然科学基金资助项目(81271088);上海市自然科学基金资助项目(11ZR1423600)


CD44 as a molecular marker to screen tumor stem cells in hypopharyngeal carcinoma
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    摘要:

    目的 探讨CD44作为筛选下咽癌肿瘤干细胞分子标记物的价值。方法 应用流式细胞仪检测下咽癌FADU细胞系中CD44的表达;采用免疫磁珠分选技术(MACS)分选CD44+细胞,并用流式细胞仪检测其纯度;运用四甲基偶氮唑蓝(MTT)比色法检测CD44+、CD44细胞的体外增殖能力,将相同数量级(1×106、1×105)的CD44+、CD44细胞分别注射于雄性非肥胖糖尿病型/重症联合免疫缺陷(nonobese diabetic/severe combined immunodefi ciency,NOD/SCID)小鼠皮下,观察其体内致瘤能力的差异。结果下咽癌FADU细胞系中CD44+细胞占(21.1±1.56)%,免疫磁珠分选后的CD44+细胞纯度达(99.4±0.29)%。体外增殖能力研究显示CD44+细胞增殖速度明显快于CD44细胞。NOD/SCID鼠体内致瘤实验结果显示:1×105CD44+细胞致瘤率为12.5%(1/8),1×105 CD44细胞致瘤率为0(0/8);1×106 CD44+细胞致瘤率为100%(8/8),1×106 CD44细胞致瘤率为50%(4/8);相同数量级的CD44+与CD44两组细胞致瘤率差异具有统计学意义(P<0.05)。此外,CD44+细胞组比CD44细胞组更早成瘤,潜伏期明显缩短。CD44+细胞组所致肿瘤体积平均为(2017.81±538.50)mm3, 而CD44细胞组所致肿瘤体积平均为(1153.25±503.18)mm3,两组差异具有统计学意义(t=2.67,P<0.05)。结论下咽癌肿瘤中CD44+细胞比CD44细胞具有更强的体外增殖能力和体内致瘤能力,提示肿瘤干细胞可能存在于CD44+肿瘤细胞内,CD44可作为筛选下咽癌肿瘤干细胞的一个重要分子标记物。

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    Abstract:ObjectiveTo study the significance of CD44 as a molecular marker for screening tumor stem cells in hypopharyngeal carcinoma.MethodsThe CD44 expression levels in the hypopharyngeal carcinoma cell line FaDu were analyzed using flow cytometry. The CD44+ and CD44cell populations were sorted by using magneticassociated cell sorting (MACS) technology. After separation, the purity of the CD44+ cells was detected using flow cytometry. The MTT method was used to detect the different proliferation capability of the CD44+ and CD44cells in vitro. The tumorigenicity of the CD44+ and CD44cells was determined by injecting CD44+ or CD44cells (1×106 and 1×105) into the body of NOD/SCID mice.ResultsCD44 expressed in (21.1±1.56)% of the FaDu cells. The CD44+ population was efficiently sorted by MACS with the purity of (99.4±0.29)%. The MTT assay indicated that the sorted CD44+ cells had a stronger proliferative capacity than the CD44cells. The tumorigenicity study showed that all the mice injected with 1×106 CD44+ cells developed tumors (8/8), half of the mice injected with 1×106 CD44cells developed tumors (4/8), 1 of the 8 mice injected with 1×105 CD44+ cells developed tumors (12.5%), but none of the mice injected with 1×105 CD44cells developed any tumors (0/8). At the same concentration, the difference in tumorigenic rates between the CD44+ and CD44groups was statistically significant (Fisher’s exact test, P< 0.05). In addition, the CD44+ group had a shorter incubation period than the CD44group. Furthermore, the average tumor volume of the CD44+ group was (2017.81±538.50) mm3, while that of the CD44group was (1153.25±503.18) mm3, and the difference was statistically significant (t=2.67, P<0.05).ConclusionsThe CD44+ cells have stronger proliferative capacity and higher tumorigenic potential than the CD44cells, which suggests that the tumor stem cells of hypopharyngeal carcinoma may exist in the CD44+ tumor cell population. Therefore,CD44 maybe an important biological marker for screening of tumor stem cells in hypopharyngeal carcinoma.

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沈晨凌 向明亮 聂琛 胡海霞 马衍 吴皓. CD44作为筛选下咽癌肿瘤干细胞分子标志物的价值[J].中国耳鼻咽喉颅底外科杂志,2013,19(5):388-392

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  • 在线发布日期: 2013-10-31
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