Abstract:ObjectiveTo explore the effect of antisense KRT13 lentivirus plasmid on the radiosensitivity of nasopharyngeal carcinoma cells (HNE1).MethodsThe lentivirus package system carrying antisense RNA of KRT13 was constructed, and stable expressed antisense KRT13 HNE1 cell line was screened by G418. The cells were randomly divided into three groups. control, lentivirus and antiKRT13 group, and to detect the content of KRT13 in each group. Cells were exposed to radiation of various dosage(0, 1, 2, 4, 6 and 8 Gy), and then the clonogenic survival and curve fitting were used for calculating the radiobiological parameters and the sensitization enhancement ratio after radiation. The apoptosis and cell cycle changes were analyzed by flow cytometry.ResultsCompared with the control cells and negative cells, antiKRT13 cells showed obviously high values of D0, Dq, N, and SF2 but significantly decreased α/βvalue and the differences were statistically significant (P<0.05). AntiKRT13 could block HNE1 cells in G2/M phase and inhibit the apoptosis.ConclusionAntisense KRT13 lentivirus plasmid can reduce the radiosensitivity of HNE1 cells in vitro.