ObjectiveTo investigate the methodology and clinical effect of islandshaped tragal cartilageperichondrium for repair of large perforation of tympanic membrane.MethodsThe composite of islandshaped tragal cartilageperichondrium was used for repair of perforation of tympanic membrane with interplantation method in 64 patients (64 ears). All patients received otoscopy and pure tone audiometry one year after operation.ResultsAll the 64 patients were followed up more than one year postoperatively. No vertigo, facial palsy or exacerbation of tinnitus occurred in all the patients. Recurrent perforation was observed in one case. The healing rate was 98.4%(63/64). Pure tone audiometry showed that the average air conduction thresholds among speech frequencies before and after myringoplasty were 38.3 dB and 29.1 dB with average air bone gap of 24.6 dB and 13.4 dB respectively. Their differences were statistically significant (P<0.05).Conclusion Myringoplasty with islandshaped tragal cartilageperichondria is a reliable method to repair perforation of tympanic membrane, especially large or recurrent perforation.