Abstract:ObjectiveTo detect in vivo radiosensitization of wogonin on transplanted nasopharyngeal carcinoma (NPC) in nude mouse, and to explore the relationship between the radiosensitization and expression of nuclear factorκB(NFκB).Methods5-8F cells were inoculated subcutaneously in the right axillas of 24 female BALB/c nude mice. When tumor nodes reached certain size (range 4-6 mm), the mice were randomly divided into four groups (6 mice for each group): control group, wogonin group (20 mg/kg, intraperitoneal injection), radiotherapy group (20 Gy), and combination group (wogonin plus radiotherapy). The tumor volume was measured once every three days. Three weeks after treatment, all mice were executed and tumors were removed and weighed. Their tumor inhibitory rates were calculated. Realtime PCR was applied to detect the expression of NFκB in tumor tissues.ResultsBoth tumor volume and weight of the combination group were less than those of the other three groups respectively (all P<0.05). Realtime PCR showed that the expression of NFκB mRNA in the combination group was decreased as compared with those of the other three groups, and the differences were all statistically significant (all P<0.05).ConclusionWogonin may increase radiosensitization of transplanted NPC in nude mice with possible mechanism of downregulated expression of nuclear factorκB.