ObjectiveTo study the effect of acoustic stimulation mode on prevalence of acoustically evoked short latency negative responses (ASNR) in children with large vestibular aqueduct syndrome (LVAS).Methods30 children (56 ears) diagnosed as LVAS with highresolution computerized tomography (HRCT) of temporal bone were included in our study. Ordinal clickABR, tone burst ABR, bone ABR tests were performed to 56 ears. The ASNR waves were recorded during the ABR test, and the prevalence was analyzed with SPSS software.Results① Of all the 56 ears, 26 (46.4%) were male and 30 (53.6%) were female, with an average age of (21.4±19.1) months (ranged from 4 months to 7 years). As for the degree of hearing loss, 22 ears（39.3%）were mild or moderate, 27（48.2%）were moderately severe, 7 （12.5%）were severe and profound. ② ASNR waves were presented in 30 ears with a total prevalence of 53.5%. ③ The prevalence of ASNR by the click stimuli was 48.2%(27/56), which was higher than those by tone burst stimuli (26.8%, 15/56) and bone stimuli (5.4%, 3/56). χ2 test showed that the differences of prevalence among different stimuli were all statistically significant (all P<0.05). The χ2 test of multiple sampling rates showed that the difference between prevalence by click stimuli and tone burst stimuli was statistically insignificant, while those between click stimuli and bone stimuli, tone burst stimuli and bone stimuli were both statistically significant.ConclusionClick stimulation mode may result in a high prevalence of ASNR. The presence of ASNR is highly indicative of LVAS.