Abstract:coherence tomography (OCT) is a real-time and non-invasive technique which can acquire high-resolution, cross-sectional images of tissue in vivo. An image produced by OCT resembles the tissue architecture observed in histology and can therefore be considered as an “optical biopsy”. As an imaging diagnostic test, OCT has been widely used in ophthalmology to image the retina and in cardio vascular applications to visualize vulnerable plaque in the coronary vasculature, as well as in urology and gastroenterology for mucosa epithelium imaging. Because the potential for OCT to discern the layered microstructure of vocal folds, OCT imaging is becoming an increasingly important technology for voice science, including detection of vocal folds structure, differential diagnosis of benign and malignant tumors, phonomicro surgery, observation of vocal folds vibration.