ObjectiveTo investigate the expression of Orai1 protein and its function in nuocytes.MethodsMice allergic rhinitis (AR) models were established using ovalbumin (OVA), and numbers of sneezing and nasal rubbing were counted. Then nuocytes were sorted and purified from the mouse nasalassociated lymphoid tissue (NALT), and the AR mice nuocytes were cultured in vitro. Qualitative examination of Orai1 protein was performed using confocal laser scanning microscopy, and quantitative examinations of this protein and its mRNA through enzymelinked immunosorbent assay (ELISA) and realtime reverse transcriptionpolymerase chain reaction (RTPCR). After that, mouse recombinant (rm) interleukin (IL)33 was administered into nuocytes cultures, and the proteins and mRNAs of IL5 and IL13 were detected. Finally, Orai1 antibody was applied into the cultures, and the two cytokines proteins and their mRNAs were reevaluated.ResultsNumbers of sneezing and nasal rubbing were increased in mice AR models. Nuocytes were identified as CD3CD4CD8CD19CD11bCD11cFcεR1 (lineage)-ICOS+, and Orai1 protein was expressed in these cells. Its protein and mRNA were enhanced in AR mice compared to normal ones. After administration of rmIL33, IL5 and IL13 proteins and mRNAs were elevated significantly in nuocytes cultures. However, their expressions were all reduced after application of Orai1 antibody.ConclusionThe intervention of Orai1 protein in nuocytes inhibits their cellular function in vitro.