ObjectiveTo evaluate the expression of Tlymphoma invasion and metastasis 1 (Tiam1) in tissue specimens of laryngeal carcinoma, and explore the correlation between Tiam1 expression and clinicopathological parameters as well as prognosis of laryngeal carcinoma.MethodsTiam1 expression in 98 primary laryngeal carcinoma tissue specimens was detected by immunohistochemistry. The correlations between Tiam1 expression and clinicopathological parameters as well as patients’ survival were analyzed.ResultsImmunohistochemical staining revealed that Tiam1 was detected in all primary tumor samples. Moreover, Tiam1 overexpression was significantly correlated with lymph node metastasis (P<0.001), clinical stage (P=0.027), histological grade (P=0.020), and recurrence (P=0.003). Survival analysis demonstrated that high Tiam1 expression was significantly correlated with shorter diseasefree survival (P=0.001) and overall survival (P<0.001). When combining the Tiam1 expression and lymph node status, KaplanMeier survival analysis showed that patients with Tiam1 overexpression/lymph node metastasis (+) had both shorter diseasefree and overall survival than others (both P<0.001). Multivariate Cox proportional hazards model analysis confirmed that lymph node metastasis (P=0.001) and Tiam1 overexpression (P=0.020) were statistically significant, independent predictors of prognosis for patients with laryngeal carcinoma.ConclusionsThe Tiam1 protein is obviously overexpressed in laryngeal carcinoma, which is associated with lymph node metastasis, recurrence, and poor prognosis of this tumor. Tiam1 may contribute to laryngeal carcinoma progression, and represent as a novel prognostic indicator as well as potential therapeutic target for laryngeal carcinoma.