Abstract:ObjectiveTo study the changes of cochlear nucleus proteomics with age in C57BL/6J mice with agerelated hearing loss and to explore the role of lower auditory centers in the pathogenesis of presbycusis.MethodsAuditory brainstem response (ABR) was used to detect the auditory thresholds of C57BL/6J mice aged 2 months and 12 months, and isobaric tags for relative and absolute quantitation (iTRAQ) and Q Exactive plus and bioinformatics were adopted to analyze the cochlear nucleus differential proteins between the two groups.ResultsA total of 5,919 proteins and 39,269 unique peptides were identified in the two groups. There were 70 differentially expressed proteins that met the screening criteria of P value (t test) less than 0.05 and whose expression difference multiple was greater than 1.2 times (updown). Among them, 44 were upregulated and 26 were downregulated.ConclusioniTRAQbased quantitative proteomics can be used effectively for tissue proteins identification and relative and absolute quantification. The differential expression profile of cochlear nucleus protein can be obtained by this technique. Further studies on the molecular mechanisms of these proteins will help to elucidate the pathogenesis of senile deafness, especially central senile deafness, and identify potential markers, thus providing new molecular targets for the diagnosis and treatment of senile deafness. Keywords: