Objective To observe the clinical effect of batroxobin combined with the conventional therapy in the treatment of patients with different types of sudden deafness. Methods Clinical data of 121 patients (135 ears) with sudden deafness were collected and analyzed. Sixty-nine cases (79 ears) in the conventional treatment group were treated with glucocorticoid, neurotrophins and vasodilators, and 52 cases (56 ears) in the batroxobin group were treated with batroxobin injection on the basis of conventional treatment. The treatment outcomes of the two groups were observed and compared after one week of treatment. Results The total effective rate of the batroxobin group was 64.29%, which was higher than that of the conventional treatment group (P=0.001). In patients with hearing curve of low-frequency descending type, the effective rate of the batroxobin group was 90.91%, which was higher than that of the conventional treatment group (P=0.149); In patients with hearing curve of high-frequency descending type, the effective rate of the batroxobin group was 60.00%, which was also higher than that of the conventional treatment group (P=0.037). In those with hearing curve of flat descending type, the effective rates of the batroxobin group and the conventional treatment group were 41.67% and 44.83%, the difference between the two groups was statistically insignificant (P=0.853). In patients with total deafness, the effective rate of the batroxobin group was 65.22%, which was higher than that of the conventional treatment group (P=0.025). Conclusion Batroxobin injection combined with the conventional treatment can effectively improve the hearing level of patients with sudden deafness, especially those with hearing curve of high-frequency descending type and total deafness.