Objective To explore the expressions of BLU, cyclin D1 and cyclin B1 and their clinical significances in nasopharyngeal carcinoma (NPC).Methods Immunohistochemical method was used to evaluate the expressions of BLU, cyclin D1 and cyclin B1 in 97 NPC tissues and 36 chronic nasopharyngitis tissues, and the relationships between the expressions of these three proteins and clinicopathological characteristic parameters as well as the prognosis of NPC patients were analyzed.Results The positive rate of BLU in NPC was lower than that in chronic nasopharyngitis, while the positive rates of cyclin D1 and cyclin B1 in NPC were higher than those in chronic nasopharyngitis (all P<0.05). BLU expression was correlated with patients’ age and T stage of tumor, while cyclin D1 expression was correlated with clinical stage and lymph node metastasis, and cyclin B1 expression was correlated with clinical stage and T stage (all P<0.05). Spearman rank correlation analysis showed that BLU was negatively correlated with cyclin D1 (r=-0.378, P=0.011) but not significantly correlated with cyclin B1 in NPC tissues (r=0.096, P=0.089), and cyclin D1 and cyclin B1 were not significantly correlated in NPC tissues (r=0.084, P=0.191). The overall survival rate of the BLU positive NPC group was higher than that of the negative group, and the overall survival rate of the cyclin D1 positive NPC group was lower than that of the negative group (P<0.05). In addition, the difference of the overall survival rate between the cyclin B1 positive group and the negative group was statistically insignificant (P>0.05). The log-rank analysis indicated that BLU, cyclin D1 and T stage as well as N stage were related to the prognosis of the NPC patients (all P<0.05). Cox regression model analysis showed that BLU, cyclin D1 and lymph node metastasis were independent predictors in the prognosis.Conclusion The down-regulated expression of BLU and up-regulated expressions of cyclin B1 and cyclin D1 may play important roles in the occurrence and development of NPC. Therefor, they may be used as markers to evaluate the prognosis of NPC patients.