Objective To analyze the results of bacterial culture and drug sensitivity test of purulent secretion of external auditory canal in patients with chronic suppurative otitis media in Chongqing, so as to provide the basis for rational perioperative drug use.Methods A total of 106 patients with chronic otitis media from Jan 2020 to May 2021 were selected as the research objects. The purulent secretion of the affected ear was taken for bacterial culture and drug sensitivity test.Results The pathogens were isolated from the specimens in 85 of the 106 patients with an isolation rate of 80.19%. Totally 107 strains of pathogens were cultured, including 51 (47.66%) strains of Gram-positive bacteria, mainly Staphylococcus aureus (19 strains, 17.76%), 27 (25.23%) strains of Gram-negative bacteria, mainly Pseudomonas aeruginosa (8 strains, 7.48%), and 29 (27.10%) strains of fungi, mainly yeast like fungi (12 strains, 11.21%). Staphylococcus aureus was highly susceptible to benzoxacillin, quinoloptin/daprotin, rifampin and vancomycin, with a sensitive rate of 100%, but highly resistant to erythromycin, penicillin and clindamycin. Pseudomonas aeruginosa was highly sensitive to ceftazidime, cefepime, imipenem, piperacillin/tazobactam, amikacin and gentamicin, with a sensitive rate of 100%, and highly resistant to ampicillin, cefazolin and compound sulfamethoxazole.Conclusions The main pathogens of chronic otitis media in Chongqing are Staphylococcus aureus, Pseudomonas aeruginosa and yeast like fungi. The climate in Chongqing is humid and the positive rate of fungi is high. Fungal infection should be paid attention to in the treatment of chronic suppurative otitis media.