Objective To investigate the efficacy of trans-tympanic catheter insertion combined with eustachian tube insufflation in treament of persistent otitis media with effusion. Methods Collected the clinical data of 98 patients(127 ears) with otitis media with effusion (OME) who were treated in our department from January 2018 to June 2020,and the clinical histories of all the patients were more than three months.The patients were divided into observation group and control group according to the random digital method. The observation group of 49 cases (59 ears) was treated with trans-tympanic catheter insertion combined with eustachian tube insufflation and the other group of 49 cases (68 ears) was treated with trans-tympanic catheter insertion alone, and compared the efficacy and complications between the two groups.Results In the observation group of 49 cases (59 ears), 55 ears were cured,3 ears were improved,the total effective rate was 98.31%(58/59), In the control group of 49 cases (68 ears), 50 ears were cured , 9 ears were improved,the total effective rate was 86.76%(59/68), the difference between the two groups was significant (P <0.05).The two groups had no adverse effects such as tympanic membrane perforation, vertigo and middle ear infection.Conclusion When the conservative treatment of persistent otitis media effusion in children is ineffective, trans-tympanic catheter insertion can be performed first. If the efficacy is not satisfactory or Chronic relapses, trans-tympanic catheter insertion combined with eustachian tube insufflation can be selected.