Objective To determine the distribution of aeroallergens in patients with allergic rhinitis (AR) in southwest Chongqing, so as to provide epidemiological evidence for the prevention and treatment of AR in this area.Methods A total of 4 199 suspected AR patients undergoing allergen test in Yongchuan Hospital of Chongqing Medical University from Jan 2017 to Aug 2020 were collected. The positive rate of each allergen was counted according to patients' age and gender, testing season,and so on. The epidemiological characteristics and trends were analyzed.Results ①Among the 4 199 suspected AR patients, there were 3 488 cases whose symptoms, signs, and intracutaneous test results were consistent, with an overall positive rate of 83.07%. ②The most common aeroallergens were dermatophagoides pteronyssinus, dermatophagoides farin, and polyvalent insects in order. ③The differences among the positive rates of allergen in different seasons were statistically significant(χ²=22.11,P<0.001), with highest positive rate in summer. ④There was significant difference of total positive rate of allergen between the male and female(χ²=10.47,P=0.001), and there was no significant correlation between most allergens and gender. ⑤With the increase of age, the positive rate of aeroallergens decreased gradually. The differences of overall positive rate were statistically significant among different age groups (all P<0.001). ⑥In terms of the number of positive allergens, patients with triple positive allergens were the most commom(11.70%).Conclusions The main allergen in southwest Chongqing is dermatophagoides pteronyssinus. The positive rate of allergen has relationship with the patients' gender and age, and the presentation season.