Objective To explore the application of acoustic immittance test combined with tympanic membrane imaging in the diagnosis of traumatic tympanic membrane perforation.Methods It was retrospectively analyzed for clinical data of 37 patients (44 ears in total) with traumatic tympanic membrane perforation received in the outpatient department of Nanjing Second Hospital from April 2019 to April 2020. The patient's medical history was detailed at the time of admission. All patients were examined by immittance instrument and tympanic membrane imaging. According to the detection method, the patients were divided into the tympanic membrane imaging group and the combined group (acoustic immittance test combined with tympanic membrane imaging). They were compared for the sensitivity, specificity and accuracy of the two groups in the diagnosis of traumatic tympanic membrane perforation.Results The sensitivity, specificity and accuracy of the tympanic membrane imaging group were 91.12%, 50.00% and 81.82%, while the sensitivity, specificity and accuracy of the combined group were 100%, 100% and 100%, respectively. Compared with the two groups, the detection methods in the combined group were higher than those in the tympanic membrane imaging group. There was statistical significance for the difference of two groups(P<0.05).Conclusions The tympanic membrane imaging alone is prone to misdiagnosis. The acoustic immittance test combined with tympanic membrane imaging has higher sensitivity, specificity and accuracy in the diagnosis of traumatic tympanic membrane perforation.