Objective To investigate the effects of two temperature test, video-head impulse test(vHIT) and vestibular autorotation test (VAT) on vestibular migraine (VM) and Meniere’s disease (MD).Methods A total of 26 patients with VM and MD were assessed by dual temperature test, vHIT and VAT. Dual temperature tests were performed to record canal paresis (CP) value and maximum slow phase velocity (SPV) in response to hot and cold stimulation on the weak side. vHIT test recorded the gain value of each semicircular canal and saccadesis; Horizontal and vertical gain as well as phase shift and asymmetrical bias were recorded by VAT examination. The differences of each examination result between the two diseases were compared by statistical analysis.Results The abnormal number of dual temperature test in VM and MD patients was 7 and 15, respectively. The CP value of VM and MD patients was(15.81±12.97)%、(28.88±15.38)%.The difference of abnormal rate (P=0.025) and CP value (P=0.002) was statistically significant. The abnormal number of vHIT test in VM and MD patients was 6 and 11, respectively. There was no statistical difference for the abnormal rate of vHIT test between the two diseases. There were 24 patients with abnormal VAT, 6 cases with increased gain, 16 cases with decreased gain, and 16 cases with delayed phase shift. There were 19 patients with abnormal VAT, 14 patients with decreased gain, and 10 patients with delayed phase shift in MD. The difference for the increase rate of gain between the two diseases was statistically significant (P=0.023). There were 1 and 8 abnormal patients in VM and MD dual-temperature test and vHIT, respectively, and the difference was statistically significant (P=0.028). There was a statistically significant difference in abnormal rate between VM patients’ dual-temperature test and VAT examination (P<0.001).Conclusions There are differences in clinical characteristics between VM and MD. MD patients are more prone to horizontal semicircular canal injury, and the degree of injury is more serious. The abnormal rate of dual temperature test and vHIT in MD patients was higher than that in VM patients, and the VAT gain of VM patients was more likely to increase. The combination of the three tests can provide evidence for the differentiation of the two diseases.