Abstract:【Abstract】 Objective The mouse model of allergic rhinitis plays an important role in the research on the occurrence and development of allergic rhinitis and the evaluation of the therapeutic effect. However, the difficulty in obtaining samples from nasal lavage and nasal mucosa in mice limits the application of this model. In this study, we improved the method to obtain nasal lavage and nasal mucosa from mice. Methods We proposed an easy method to obtain the nasal lavage of mouse through the postnasal way and compared it with the method usually used through tracheal way. In addition, we proposed two methods for obtaining the nasal mucosa of mice and demonstrated the process in the form of pictures. Results The recovery of lavage fluid collected by the simple-trained experimenter using the postnasal way was significantly higher than that obtained by the experienced experimenter using the tracheal way. and the average recovery rate of nasal lavage fluid was up to 93% in this new method, and the operation time is significantly shortened. In the process of obtaining the nasal mucosa of mice, the two methods proposed in this study can respectively achieve the maximum obtaining of the nasal mucosa of mice or obtain the nasal mucosa from different locations. Conclusion The methods to obtain nasal lavage and the nasal mucosa of mice proposed in this study are simple to operate, efficient and easy to grasp, and will provide an important reference for the study of the mouse model of allergic rhinitis.