Abstract:Objective The mouse model of allergic rhinitis plays an important role in the research on the occurrence and development of AR and the evaluation of the therapeutic effect. However, the difficulty in obtaining samples from nasal lavage and nasal mucosa in mice limits the application of this model. In this study, we improved the methods to obtain nasal lavage fluid and nasal mucosa from mice.Methods An easy method to obtain the nasal lavage fluid of mouse through the postnasal way was proposed and compared with the method through tracheal way. In addition, two methods (i.e. dual windows through nasal bone and palate, subsite obtainment) were proposed for obtaining the nasal mucosa of mice and the processes were demonstrated in the form of pictures.Results The recovery of lavage fluid collected by the simple-trained experimenter using the postnasal way was significantly higher than that obtained by the experienced experimenter using the tracheal way. The average recovery rate of nasal lavage fluid was up to 93% in this new method, and the operation time was significantly shortened. In the process of obtaining the nasal mucosa of mice, the two methods proposed in this study could achieve the maximum obtainment of nasal mucosa or nasal mucosa from different locations respectively.Conclusion The methods to obtain nasal lavage fluid and the nasal mucosa of mice proposed in this study are simple to operate, efficient and easy to grasp, and will provide an important reference for the study of the mouse model of AR.