Objective To investigate the key targets of curcumin against laryngeal cancer based on network pharmacology and to design experiments to verify them.Methods Curcumin targets were screened by Swiss Target Prediction, laryngeal cancer targets were screened by GeneCards, OMIM and TTD databases.The common targets of curcumin-laryngeal cancer were obtained by FunRich softwar.Gene ontology biological processes and Kyoto Encyclopedia of Genes and Genomes pathway analysis of curcumin-laryngeal cancer common targets were performed using DAVID platform.Cytoscape software was used to screen key targets.The key targets were verified and analyzed by molecular docking.The effect of curcumin on the migration ability of laryngeal cancer cells was observed by cell scratching assay. Cell counting kit (CCK-8) was used to detect the effect of curcumin on cell proliferation and the synergistic effect of curcumin on doxorubicin. Western blot was used to detect the effect of curcumin on the expression of phosphorylated signal transduction and transcription factor 3 (P-STAT3) protein in laryngeal carcinoma cells.Results Twelve shored targets were obtained. KEGG pathway enrichment analysis revealed that the signaling pathways related to laryngeal cancer were proteoglycan pathway in cancer, tumor necrosis factor (TNF) signaling pathway, pathway in cancer, hypoxia inducing factor 1 (HIF-1) signaling pathway, Jak-STAT signaling pathway, carbon metabolism pathway in cancer, and vascular endothelial growth factor signaling pathway.By molecular docking,it was found that: curcumin binds well to P-STAT3.It was observed that curcumin could inhibit the migration ability of laryngeal cancer cells by cell scratch assay.CCK-8 method found that curcumin could significantly inhibit the proliferation activity of laryngeal cancer cells in vitro, and curcumin had a synergistic effect on doxorubicin. Western blot assay showed that the increase of curcumin concentration could inhibit the expression of p-STAT3 protein in laryngeal cancer cells.Conclusions Curcumin inhibited the proliferation and migration of laryngeal cancer cells in a dose-dependent manner. Curcumin had a synergistic effect on doxorubicin. curcumin inhibited laryngeal cancer tumor cells by regulating EGFR, EP300 and p-STAT3, and the validated target was p-STAT3.