Abstract:Objective To study the distribution of fungal species in otomycosis and to detect the drug sensitivity of pathogenic bacteria to antifungal drugs. Methods From April 2020 to November 2021, 239 patients with otomycosis who were treated in the Otorhinolaryngology Department of Jingzhou Central Hospital were collected. Fungal species were identified by morphological examination, sand glucose solid medium (SDA) fungal culture, DNA extraction, PCR amplification and sequencing in ITS region. Minimum inhibitory concentration (MIC) of bibenbenzazole (PCB), terbinafine (TRB), clotrimazole (CLO) and itraconazole (ITR) were determined by minimum inhibitory concentration test. Results Aspergillus was the most common pathogen of otomycosis, followed by Aspergillus Niger, Aspergillus flavus and candida. Most of the pathogenic bacteria causing otomycosis were resistant to PCB and CLO (MIC > 16μg/ mL), and most of the pathogenic bacteria were sensitive to TRB and ITR. Therefore, ITR and TRB drugs should be the first choice in the treatment of otomycosis. Conclusion Aspergillus and Candida are the most common fungal pathogens of otomycosis. ITR and TRB are the first choice drugs for the treatment of otomycosis.