1.Guangxi University of Chinese Medicine;2.Liuzhou People’s Hospital
目的 探讨基质金属蛋白酶2（Matrix metalloproteinase-2, MMP-2）、基质金属蛋白酶9（Matrix metalloproteinase-9, MMP-9）及胃蛋白酶（Pepsin）对喉鳞状细胞癌局部侵袭的作用。方法 收集2020年10月至2022年3月柳州市人民医院耳鼻咽喉科住院手术并经病理确诊的32例喉鳞状细胞癌患者的癌组织和癌旁组织新鲜标本，同期收集29例成人咽喉乳头状瘤组织及瘤旁组织新鲜标本，通过实时荧光定量PCR（qRT-PCR）分别检测MMP-2、MMP-9及Pepsin mRNA在上述组织标本中的表达，结合临床资料分析。结果 喉癌组织中MMP-2、MMP-9及Pepsin mRNA表达高于癌旁组织，差异具有统计学意义（P＜0.05）；III、IV期组表达水平高于I、II期组（P＜0.05），淋巴结转移组高于无转移组（P＜0.05）；MMP-2、MMP-9 mRNA在中-低分化鳞状细胞癌组的表达高于高分化组（P＜0.05）；Pepsin mRNA在60岁及以上喉癌组中表达高于60岁以下组（P＜0.05）；MMP-2、MMP-9及Pepsin mRNA在喉癌组织表达均高于乳头状瘤组和瘤旁组织组（P＜0.05），且从瘤旁组织、乳头状瘤组织到喉癌组织，MMP-2、MMP-9及Pepsin mRNA表达水平有升高趋势。结论 MMP-2、MMP-9和Pepsin在喉鳞状细胞癌的局部侵袭过程中发挥协同促进作用，预防和治疗咽喉反流是防止喉癌局部侵袭的重要环节。
Objective: To investigate the effect of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and pepsin on local invasion of laryngeal squamous cell carcinoma. Methods: From October 2020 to March 2022, fresh specimens of cancer tissue and paracancerous tissue from 32 patients with laryngeal cancer who underwent inpatient surgery in the otolaryngology department of Liuzhou People's Hospital and were pathologically diagnosed were collected. During the same period, fresh specimens of 29 adult pharyngeal papilloma tissues and adjacent tissues were collected. The expressions of MMP-2, MMP-9 and Pepsin mRNA in the above tissue samples were detected by real-time fluorescence quantitative PCR (qRT-PCR), and analyzed in combination with clinical data. Results: The expression of MMP-2, MMP-9 and Pepsin mRNA in laryngeal carcinoma tissues was higher than that in adjacent tissues (P<0.05). The expression level in stage III and IV groups was higher than that in stage I and II groups (P<0.05), and that in lymph node metastasis group was higher than that in non-metastasis group (P<0.05). The expression of MMP-2 mRNA and MMP-9 mRNA in medium-low differentiated squamous cell carcinoma group was higher than that in well differentiated group (P<0.05); the expression of Pepsin mRNA in laryngeal carcinoma group aged 60 years or older was higher than that in laryngeal carcinoma group aged less than 60 years (P<0.05); the expression of MMP-2 mRNA, MMP-9 mRNA and Pepsin in laryngeal carcinoma tissue was higher than that in papilloma and adjacent tissue group (P<0.05). In addition, the expression levels of MMP-2, MMP-9 and Pepsin mRNA increased from paratumoral tissue, papilloma tissue to laryngeal carcinoma tissue. Conclusion: MMP-2, MMP-9 and Pepsin play a coordinated role in local invasion of squamous carcinoma of the larynx. Prevention and treatment of laryngopharyngeal reflux is an important part of preventing the local invasion of laryngeal carcinoma.