Abstract:[Abstract] Objective: The purpose of this paper is to screen children with seasonal allergic rhinitis(AR)for allergen components and understand the allergenic compents of pollen in children with AR. Methods:Artemisia annua and sieversiana artemisia pollen were collected and pollen proteins were extracted to screen for allergenic components in pollen with clinically confirmed AR and special immunoglobulin E (sIgE)tested positive for artemisia.Screening of allergen components in pollen by western blotting. Results:Western blotting showed that three main bands of the two Artemisia plant pollen protein extracts specifically bind to serum IgE, with relative molecular mass(Mr)is12, 25, and 70 kDa, respectively.The 70 kDa allergen in Artemisia annua pollen was the most common allergen molecule in children with AR,with a positive detection rate of 85.19%, 25 kDa and 12 kDa allergens of 37.04% and 14.81%, respectively. The positive detection rate of 70 kDa allergen in Artemisia sieversiana was lower than that of Artemisia annua (P<0.05) at 51.85%, and the positive detection rate of 25 kDa and 12 kDa allergen was similar to that of Artemisia scutellaria (P>0.05). In addition, there were differences in the positive response of the same body serum to the two artemisia homologous allergens, and different children were sensitive to one or more allergen molecules. Conclusion: In children with AR in this region, the sensitization rate of Artemisia annua pollen is higher, and it is recommended to increase the pollen component protein of Artemisia scutellaria for diagnosis and immunotherapy, especially the 70 kDa allergen molecule.