变应性鼻炎儿童蒿花粉变应原组分诊断
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1.内蒙古自治区妇幼保健院;2.内蒙古自治区妇幼保健院儿内科;3.内蒙古医科大学研究生学院;4.内蒙古医科大学公共卫生学院

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内蒙古自治区自然科学基金,基金编号:2020MS08200


Diagnosis and sensitization characteristics of artemisia pollen allergens in children with allergic rhinitis
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    摘要:

    本文旨在对季节性变应性鼻炎(AR)患儿进行变应原组分筛查,了解AR患儿代表性蒿属植物花粉变应原致敏组分。方法:采集黄花蒿和大籽蒿花粉,提取花粉蛋白,以临床确诊为AR且艾蒿特异性免疫球蛋白E(sIgE)检测为阳性患儿血清为一抗,通过蛋白质免疫印迹法筛查花粉中变应原组分。结果:蛋白质免疫印迹显示两种蒿属植物花粉蛋白提取物中主要有三个条带能与血清IgE特异性结合,相对分子量(Mr)分别为12、25和70 kDa。黄花蒿花粉中70 kDa变应原为AR儿童最常见的变应原分子,阳性检出率为85.19 %,25 kDa和12 kDa变应原分别为37.04%和14.81%。大籽蒿花粉中70 kDa变应原阳性检出率低于黄花蒿(P<0.05),为51.85%,25 kDa和12 kDa变应原阳性检出率与黄花蒿相似(P>0.05)。另外,同一个体血清对两种蒿属植物同源变应原阳性反应存在差异,不同患儿对一种或多种变应原分子敏感。结论:AR患儿黄花蒿花粉致敏率更高,推荐增加黄花蒿花粉组分蛋白进行诊断和免疫治疗,尤其是70 kDa变应原分子。

    Abstract:

    [Abstract] Objective: The purpose of this paper is to screen children with seasonal allergic rhinitis(AR)for allergen components and understand the allergenic compents of pollen in children with AR. Methods:Artemisia annua and sieversiana artemisia pollen were collected and pollen proteins were extracted to screen for allergenic components in pollen with clinically confirmed AR and special immunoglobulin E (sIgE)tested positive for artemisia.Screening of allergen components in pollen by western blotting. Results:Western blotting showed that three main bands of the two Artemisia plant pollen protein extracts specifically bind to serum IgE, with relative molecular mass(Mr)is12, 25, and 70 kDa, respectively.The 70 kDa allergen in Artemisia annua pollen was the most common allergen molecule in children with AR,with a positive detection rate of 85.19%, 25 kDa and 12 kDa allergens of 37.04% and 14.81%, respectively. The positive detection rate of 70 kDa allergen in Artemisia sieversiana was lower than that of Artemisia annua (P<0.05) at 51.85%, and the positive detection rate of 25 kDa and 12 kDa allergen was similar to that of Artemisia scutellaria (P>0.05). In addition, there were differences in the positive response of the same body serum to the two artemisia homologous allergens, and different children were sensitive to one or more allergen molecules. Conclusion: In children with AR in this region, the sensitization rate of Artemisia annua pollen is higher, and it is recommended to increase the pollen component protein of Artemisia scutellaria for diagnosis and immunotherapy, especially the 70 kDa allergen molecule.

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  • 收稿日期:2022-10-14
  • 最后修改日期:2022-11-15
  • 录用日期:2022-11-21
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