Objective: To study the characteristics of Mismatch negativity (MMN) in children with normal hearing, and to analyze the differences of MMN in children with different ears and genders. Methods: A total of 44 children (25 males and 19 females) with normal pure tone audiometry and auditory brainstem response were examined by MMN in both ears. Classical Oddball mode was adopted. Standard and deviated stimuli were 1000 Hz and 2000 Hz, respectively. The characteristics of MMN latency and amplitude in children with different genders and ears were observed and compared. Results: MMN waveform could be elicited from both ears of 44 subjects. The average latency and amplitude of MMN in male children was 170.24±32.83ms, 3.40±2.04uV. The average MMN latency and amplitude of male children was 172.51±24.19ms,4.20±2.29uV. The average MMN latency of female children was 162.13±31.40ms, and the average amplitude was 4.01±2.82uV. The average MMN latency of female children was 170.30±33.11ms, and the average amplitude of MMN was 3.76±2.80uV. There were no significant differences in MMN latency and amplitude between the left and right ears of all subjects (p>0.05); There was no significant difference in MMN latency between the left and right ears of males (p>0.05); The MMN amplitude of male left and right ears was statistically different (p<0.05); There were no significant differences in MMN latency and amplitude between left and right ears (p>0.05). There were no significant differences in MMN latency and amplitude between males and females (p>0.05). Conclusion : Children with normal hearing can stably elicit MMN waveforms under the stimulation of short pure tone with different frequency. In children, gender and different ears have no significant influence on the latency and amplitude of MMN. However, gender cannot be excluded as a factor affecting the standard data of MMN examination, and further study is needed to expand the sample size.