目的:探究趋化因子受体4(CXCR4)及趋化因子配体12(CXCL12)在下咽鳞状细胞癌(HSCC)患者中的表达及其临床意义。方法:以本院2017年1月至2022年11月收治的90例HSCC患者为研究对象,以HSCC患者手术切除的HSCC组织、癌旁组织为实验材料。采用免疫组织化学染色法检测HSCC组织和癌旁组织中CXCR4、CXCL12的表达情况；采用实时荧光定量PCR(qRT-PCR)法检测HSCC组织和癌旁组织中CXCR4 mRNA、CXCL12 mRNA水平；收集并记录HSCC患者临床病理特征,分析CXCR4、CXCL12表达水平与患者临床病理特征的关系；采用Pearson分析CXCR4与CXCL12水平相关性。结果:CXCR4在HSCC组织中的高表达率为60.00 %,明显高于癌旁组织(17.78 %)(P<0.05)；CXCL12在HSCC组织中的高表达率为57.78 %,明显高于癌旁组织(24.44 %)(P<0.05)。HSCC组织中CXCR4、CXCL12水平均与淋巴结是否转移、TNM分期、分化程度有关(P<0.05)。与癌旁组织相比较,HSCC组织CXCR4、CXCL12水平均显著升高(P<0.05)。Pearson分析显示,HSCC患者中CXCR4水平与CXCL12水平呈正相关(r=0.538,P<0.05)。结论:CXCR4、CXCL12在HSCC组织中呈高表达,两者与HSCC淋巴结转移、分化程度、TNM分期有关。
Objective: To investigate the expression and clinical significance of chemokine receptor 4 (CXCR4) and chemokine ligand 12 (CXCL12) in hypopharyngeal squamous cell carcinoma (HSCC). Methods: Ninety cases of HSCC patients admitted to our hospital from January 2017 to November 2022 were taken as the study objects, and the HSCC tissues and adjacent tissues of HSCC patients were taken as the experimental materials. Immunohistochemical staining was applied to detect the expression of CXCR4 and CXCL12 in HSCC tissues and adjacent tissues; the levels of CXCR4 mRNA and CXCL12 mRNA in HSCC tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR); the clinicopathological characteristics of HSCC patients were collected and recorded, the relationship between CXCR4 and CXCL12 expression levels and the clinicopathological characteristics of patients was analyzed; Pearson was applied to analyze the correlation between CXCR4 and CXCL12 levels. Results: The high expression rate of CXCR4 in HSCC tissues was 60.00%, which was obviously higher than that in adjacent tissues (17.78%) (P<0.05); The high expression rate of CXCL12 in HSCC tissues was 57.78%, which was obviously higher than that in adjacent tissues (24.44%) (P<0.05). The levels of CXCR4 and CXCL12 in HSCC tissue were related to lymph node metastasis, TNM stage and differentiation (P<0.05). Compared with the adjacent tissues, the levels of CXCR4 and CXCL12 in HSCC tissues were obviously higher (P<0.05). Pearson analysis showed that CXCR4 level was positively correlated with CXCL12 level in HSCC patients (r=0.538, P<0.05). Conclusion: CXCR4 and CXCL12 are highly expressed in HSCC tissues, which are related to lymph node metastasis, differentiation degree and TNM stage of HSCC.