Objective To investigate the situation of serum 25-hydroxy vitamin D levels in patients with benign paroxysmal positional vertigo (BPPV), and to explore the possible correlations among BPPV, gender, age, and seasonality.Methods This retrospective study consisted of 208 patients with BPPV in Beijing Chaoyang Hospital from Oct 2018 to Sep 2021.Results The median serum 25(OH)D level was 15.31 (10.68, 21.03) ng/mL. The percentages of vitamin D deficiency, insufficiency, and sufficiency were 70.19%, 19.71%, and 10.10%, respectively. The serum 25(OH)D level in male patients was higher than that in females, and it was higher in the 61-70 years old group than in other age groups. The serum 25(OH)D level was the highest in summer, and significantly higher in summer and autumn than in winter and spring. Multivariate regression analyses revealed that gender, age, and season were associated with serum 25(OH)D level.Conclusion Vitamin D deficiency is prevalent in BPPV patients in Beijing. Serum 25(OH)D level may be affected by gender, age, and season.