Objective To introduce a surgical method repairing tympanic membrane with biological gel-assisted air suspension technology, and observe the eardrum healing process.Methods Patients diagnosed tympanic membrane perforation in Nanjing Benq hospital from February to June in 2023 were enrolled in this study. There were 24 cases (25 ears) of patients, including 13 males and 11 females, aged (42.3±10.1) years old. Biological gel-assisted air suspension technology were used to repairing tympanic membrane.Otologic endoscopy examination were used 3 days, 6 days, 2, 4, 8 and 12 weeks after operation.Results The operation time of the case was (34.2±10.5) minutes.Perforations were found in 2 ears 3 days after operation,there was infection in one case 3 weeks after operation, There were no bleeding, ear stuffy and allergy.All of the tympanic membranes healed well in the following up.Conclusions Biological gel-assisted air suspension technology is a simple, minimally invasive method for tympanic membrane repairing. It provides a new option for the the only hearing ear and convenience for simultaneous surgery of two sides, local anesthesia surgery and children who cannot coordinate with postoperative management.